Olympic B3 Science Summer Camp 2012
  Biotechnology, Biodiversity and Bioinformatics

  June 11th - June 30th

Schedule:
(Subject to change)

Date - June
9am-10am
10am-11am
11am-12:00pm
12:00pm-12:30pm
12:30pm-2pm
11th Putnam and Smith
Getting Acquainted Activity
Intro to lab:
(1)Establish rules, and set expectations.
(2) Lab teams meet at assigned station.
(3)Lab coats
Smith
Pre-Assessment activities
Weller
Plant Biology
The Plant Game (generate some data)
Lunch Break
Note: You will need to bring your own lunch today.
Smith
(1)Pipetting skills (2) Group Picture
12th Weller -
(1) Using Excel to format Data
(2)Pathogen Biology
Putnam
(1) Meiosis and Variation
(2) Computational Activity: Punnett Squares
Smith
Lab Skills- using balances
(1)Weighing volumes (2) Spectrophotometry on diluted solutions
Lunch Break

Note: You will need to bring your own lunch today.
Smith
(1)Experimental Design: sampling, labelling, preservation
(2) Continue morning Lab Skill activities
13th Weller
(1)Learning Box of Chestnut Material
(2)Graphing Results in Excel
Ranger Kelly Cooke
Natural History of Crowder's Mountain - Biogeography
Ranger Kelly Cooke
The Chestnut Blight
Lunch Break
Note: You will need to bring your own lunch today.
Smith
(1) Rules for the field trip - lists, times and places (2) Methods for Recording Observations
June 14th
Please arrive at the parking lot by 8:15am
Wear proper clothing and bring lots of water and your lunch!

If you are arriving on your own, please arrive at the Linwood site by 9:15am.

Directions: Take I 85 South (through Gastonia) to Edgewood Road (Exit 13).Take a left (south) and cross over Highway 74. Continue straight.This will be Archie Whitesides Road. Continue until it dead ends into Linwood Rd.Turn left. The Linwood Access and parking area will be on the right - just a short distance down Linwood. Map
15th Weller
(1) Using Excel to graph The Plant Game data
Steve Barilovits
The American Chestnut Foundation - breeding and testing for resistance to two blights
Steve Barilovits
Practical demonstration of hybrids (2) Smith: spectrophotometry
Lunch Break
Smith
Lab skills: dilutions and spectrophotometry
18th Weller and Putnam
(1) Explaining the DNA extraction protocol through a virtual lab (2) Chemistry - how selective extractions work
All
Lab skills - grinding plant tissues
All
Lab skills- using centrifuges
Lunch Break
Smith
Lab Skills- removing cell debris by centrifugation
19th Weller
(1)Introduction to algorithms
(2) More graphing with Excel (functions and slopes)
Putnam
Fragmentation of DNA: restriction enzymes and nucleases
Smith
Lab: selectively solubilize DNA, add RNAse.
Lunch Break
Smith and Putnam
Lab: Column purify the DNA and quantify it
20th Weller
How much DNA you purified: using Beer's Law
Smith
Restriction Enzymes
Putnam
(1) Lab Skills: Set up restriction digestions.
(2) Bacterial DNA extraction (Group 2)
Lunch Break
Smith
(1)Lab Skills: Mechanics of pouring agarose gels
(2) Continue DNA purification
21st Weller
Image analysis programs for gel electrophoresis
Putnam
(1) Basics of Gel Electrophoresis
(2) Metagenomics
Smith
Lab Skills: Load agarose gels with samples and standards, run over lunch
Lunch Break
Smith
Lab Skills: Stain gels and document the results
June 22nd
Field trip to the North Carolina Research Campus (NCRC) in Kannapolis
Host: Dr. Ann Loraine

24th Weller
Analysis of the gel results
Interpretation of DNA fingerprints
Putnam
The polymerase chain reaction
Smith
Lab Skills: Setting up Master Mixes for RAPD PCR reactions
Programming the thermocyclers
Lunch Break Putnam
Pour agarose gels and prepare samples for loading
25th Weller
Designing PCR primers for specific pieces of DNA
Putnam
Lab skills: load samples and markers on gels, run gels
Smith
Lab Skills: Set up a gene-specific PCR reaction
Lunch Break Smith
Lab Skills: stain and document gels - use class time to analyse patterns
26th Weller
Scoring different types of molecular markers
Putnam
Pour and load agarose gels for gene-specific PCR products
Smith
Methods for inserting DNA into foreign cells
Lunch Break
Putnam
Stain, image gels and analyse the gene-specific products
27th Weller -
Finding matches to DNA sequence: the BLAST algorithm.
Putnam
The chemistry of sequencing reactions
Weller
Making sequencing libraries
Lunch Break
Smith
Lab skills: fragment DNA non-enzymatically
28th
Parents Night - 4:30-5:30pm - Please come see our results!!!
Weller
Bioethics and the ethical, legal and social implications (ELSI) of the Human Genome projects
Weller
Ethics scenarios for class discussion
Putnam
Prepare stations for demos for parents
Lunch Break
Smith
Prepare posters of results for parents
June 29th
Field trip to the UNC Charlotte Campus
Host: Dr. Jennifer Weller

Presentations
Lists
Extra Reading
Web Links
Discussion Questions
Lab Protocols
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