Olympic B3 Science Summer Camp 2013
Biotechnology, Biodiversity and Bioinformatics
June 13th - June 28th
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Schedule:
(Subject to change)
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Date - June
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9am-10am
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10am-11am
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11am-11:30pm
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11:30pm-12:00pm
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12:00pm-2pm
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| 13th
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Putnam and Smith
Getting Acquainted Activity
Intro to lab:
(1)Establish rules, and set expectations.
(2) Lab teams meet at assigned station.
(3)Lab equipment described
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Smith
Pre-Assessment activities
Guest: ACF Treasurer Steve Barilovits: Hybrid trees, Learning Box of Chestnut Materials
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Guest: Ranger Kelly Cook
Natural History of Crowder's Mountain - Biogeography
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Lunch Break
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Guest: Ranger Kelly Cook
The Chestnut Blight
Ms. Smith Preparation notes for field trip - collecting/recording team duties
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June 14th
Please arrive at the parking lot by 8:15am
Wear proper clothing and bring lots of water, sun screen, and your lunch!
If you are arriving with a parent, please be sure to arrive at the Linwood site by 9:15am.
Directions: Take I 85 South (through Gastonia) to Edgewood Road (Exit 13).Take a left (south) and cross over Highway 74. Continue straight.This will be Archie Whitesides Road.
Continue until it dead ends into Linwood Rd.Turn left. The Linwood Access and parking area will be on the right - just a short distance down Linwood.
Map
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| 17th
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Weller -
(1) Overview of lab activities
(2)Making Lab solutions - how to read and carry out the instructions
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Weller, Smith, Putnam
Lab Skills : accuracy and precision when pipetting
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Weller, Smith, Putnam
Lab Skills: mixing and centrifuging solutions
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Lunch Break
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Guest Speaker: Peter Garibaldi, Chief Horticulturist of Stowe Botanical Gardents
Biodiversity in a show garden
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| 18th
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Weller
(1)First steps of the DNA purification method explained and demonstrated
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Weller, Smith, Putnam
Tissue grinding and incubation
Putnam
(1) Plant Breeding: Meiosis and Variation
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Weller, Smith, Putnam
DNA extraction and centrifugation
Putnam
(2) Computational Activity: Punnett Squares
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Lunch Break
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Weller, Smith, Putnam
Separate phases, precipitate DNA
Weller
Biotech Careers; Ethics example of the day
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| 19th
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Weller
Continuation of DNA purification explained and demonstrated
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Weller, Smith, Putnam
Pellet DNA, resuspend, remove RNA
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Smith
Spectrophotometry and dilutions
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Lunch Break
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Weller, Smith, Putnam
Practice dilutions, spectrophotometry, Final DNA precipitation
Weller
Biotech Careers; Ethics example of the day
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| 20th
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Smith
How to make sure you have clean DNA: spectrophotometry, gel electrophoresis
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Smith, Putnam
Pellet and resuspend DNA, use spectrophotometer
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Smith, Putnam
Lab skills- pour and load agarose gels
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Lunch Break
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Smith, Putnam
Stain agarose gels and make a digital image
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June 21st
Field trip to the North Carolina Research Campus (NCRC) in Kannapolis
Hosts: Dr. Ann Loraine and Dr. Charles David
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| 24th
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Weller
The Polymerase chain reaction explained and demonstrated
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Weller, Smith, Putnam
Set up PCR reactions, pour agarose gels
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Weller, Smith, Putnam
Put PCR reactions on agarose gels
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Lunch Break
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Weller, Smith, Putnam
Stain agarose gels, make a digital image
Weller
Biotech Careers; Ethics example of the day
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| 25th
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Weller
Interpreting the agarose gels - problem solving
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Weller, Smith, Putnam
Additional DNA purification, additional PCR reactions
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Weller, Smith, Putnam
Continue problem solving
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Lunch Break
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Weller, Smith, Putnam
Continue problem solving
Weller
Biotech Careers; Ethics example of the day
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| 26th
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Weller
Interpretation of DNA fingerprints
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Weller, Smith, Putnam
Final lab experiments to fill in results
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Weller, Smith, Putnam
Carry out data analysis, write up reports
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Lunch Break
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Putnam
Plan posters and team demonstrations for Parents Day
Weller
Biotech Careers; Ethics example of the day
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27th
Parents Show and Tell - 12:30-2:00pm - Please come see our results!!!
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Smith
Prepare posters of results for parents
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Putnam
Prepare stations for demos for parents
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Weller
Ethics Discussion - recombinant DNA in food crops
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Lunch Break - welcome Parents
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All
Show Parents what you have learned, Celebrate and see certificates awarded
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28th
Possible Field trip to the UNC Charlotte Campus (contigent on transportation)
Host: Dr. Jennifer Weller
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Presentations
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Lists
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Extra Reading
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Web Links
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Discussion topics
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Lab Protocols
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