Olympic B3 Science Summer Camp 2014
  Biotechnology, Biodiversity and Bioinformatics

  June 11th - June 26th

Schedule:
(Subject to change)

Date - June
9am-10am
10am-11am
11am-11:30pm
11:30pm-12:00pm
12:00pm-2pm
11th Putnam and Smith
Getting Acquainted Activity
Intro to lab:
(1)Establish rules, and set expectations.
(2) Lab teams meet at assigned station.
(3)Lab equipment described
Smith
Pre-Assessment activities

Guest: ACF Treasurer Steve Barilovits: Hybrid trees, Learning Box of Chestnut Materials
Guest: Ranger Kelly Cook
Natural History of Crowder's Mountain - Biogeography
Lunch Break

Guest: Ranger Kelly Cook
The Chestnut Blight

Ms. Smith and Dr. Weller Preparation notes for field trip - collecting/recording team duties
June 12th - Field Trip to Pryor Farm, at 72 Will Hill Road, Hendersonville, NC.
Route via US 74 West, I-26 West, and US 64 East.
Please arrive at the parking lot by 7:15am
Wear proper clothing and bring lots of water, sun screen, and your lunch!

If you are traveling separately, please be sure to check your map and have the cell phone of one of the teachers.

Directions from Olympic High School. Head North on Sandy Porter Rd and take the first right onto Arrowood Rd, in 0.4mi turn Left onto 485 Inner North (to Gastonia). Take exit 10 for I-85 toward Gastonia. After 20 miles take exit 10B to get on I74W towards Kings MT. After 56miles merge onto I-26W. After 16 miles take exit 49A to merge onto US-64E, toward the Bat Cave. Turn Right onto Gilliam MT Road, then Left onto Sugarloaf MT Road, first Left onto Ledbetter Rd, which dead ends at Bald Rock Rd, where you take a slight Left. Bald Rock more or less dead-ends - take a Right onto Blythe Stepp Rd, the second left onto Timber Trail and continue onto Will Hill Rd. The destination will be on the right. A Hand-drawn map of the last bit is linked here.
PSisco Directions and from Google Maps GoogleMaps Directions
June 13th - Crowders Mt State Park Collecting Trip
Please arrive at the parking lot by 8:15am
Wear proper clothing and bring lots of water, sun screen, and your lunch!

If you are traveling separately, please be sure to arrive at the Linwood parking lot by 9:15am.

Directions: Take I 85 South (through Gastonia) to Edgewood Road (Exit 13).Take a left (south) and cross over Highway 74. Continue straight.This will be Archie Whitesides Road. Continue until it dead ends into Linwood Rd.Turn left. The Linwood Access and parking area will be on the right - just a short distance down Linwood.
Map of Crowders MT SP
16th Weller -
(1) Overview of lab activities
(2)Making Lab solutions - how to read and carry out the instructions
Weller, Smith, Putnam
Lab Skills : accuracy and precision when pipetting
Weller, Smith, Putnam
Lab Skills: mixing and centrifuging solutions
Lunch Break

Putnam
Keeping a laboratory notebook, using the microscope and activities available while waiting for long steps in experiments.
17th Weller
(1)First steps of the DNA purification method explained and demonstrated
Weller, Smith, Putnam
Tissue grinding and incubation
Putnam
(1) Plant Breeding: Meiosis and Variation
Weller, Smith, Putnam
DNA extraction and centrifugation
Putnam
(2) Computational Activity: Punnett Squares
Lunch Break
Weller, Smith, Putnam
Separate phases, precipitate DNA
Weller
Biotech Careers; Ethics example of the day
18th Weller
Continuation of DNA purification explained and demonstrated
Weller, Smith, Putnam
Pellet DNA, resuspend, remove RNA
Smith
Spectrophotometry and dilutions
Lunch Break
Weller, Smith, Putnam
Practice dilutions, spectrophotometry, Final DNA precipitation
Weller
Biotech Careers; Ethics example of the day
19th Smith
How to make sure you have clean DNA: spectrophotometry, gel electrophoresis
Smith, Putnam
Pellet and resuspend DNA, use spectrophotometer
Smith, Putnam
Lab skills- pour and load agarose gels
Lunch Break
Smith, Putnam
Stain agarose gels and make a digital image
June 20th Sequencing Lab Field Trip
Field trip to the Bioinformatics Building on the UNC Charlotte campus
Hosts: Dr. Jennifer Weller and Dr. Cathy Moore
Please arrive at the parking lot by 8:15am
Wear proper clothing for a research lab and bring your lunch.
If you are traveling separately, please be sure to check your map and have the cell phone of one of the teachers.
Directions from Olympic HS to Bioinformatics UNC Charlotte
23rd Weller
The Polymerase chain reaction explained and demonstrated
Weller, Smith, Putnam
Set up PCR reactions, pour agarose gels
Weller, Smith, Putnam
Put PCR reactions on agarose gels
Lunch Break
Weller, Smith, Putnam
Stain agarose gels, make a digital image
Weller
Biotech Careers; Ethics example of the day
24th Weller
Interpreting the agarose gels - problem solving
Weller, Smith, Putnam
Additional DNA purification, additional PCR reactions
Weller, Smith, Putnam
Continue problem solving
Lunch Break
Weller, Smith, Putnam
Continue problem solving
Weller
Biotech Careers; Ethics example of the day
25th Weller
Interpretation of DNA fingerprints
Weller, Smith, Putnam
Final lab experiments to fill in results
Weller, Smith, Putnam
Carry out data analysis, write up reports
Lunch Break Putnam
Plan posters and team demonstrations for Parents Day
Weller
Biotech Careers; Ethics example of the day
26th
Student Demonstration Day - 6-8:30pm - Please come see our results!!!
Smith
Prepare posters of results for parents
Putnam
Prepare stations for demos for parents
Weller
Ethics Discussion - recombinant DNA in food crops
Lunch Break
With Guests: 6-8:30pm at Steele Creek Community Room, 13640 Steelecroft Cr. Charlotte, NC 28278
Show your family and friends what you have learned, Celebrate and see certificates awarded
Directions
Presentations
Pictures and Blog
Extra Reading
Web Links
Discussion topics
Lab Protocols
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