We will post descriptions of procedures, images that we think will help students recognize equipment and details that are easy to forget, and other aids, such as practice sets for
carrying out calculations, tutorials and so on. This page will be updated frequently.
June 12 th
- Understanding Stock Solutions of Reagents Link
Comments
The skills include keeping good notebooks, how to collect and label samples, lab equipment recognition and safe use, general assignment of benches, reminder of safe lab practices.
June 16 th
- Diluting stocks, vortexing,centrifuging small volumes Link
- Pipetting Small Volumes accurately Link
Comments
The skills include using micro pipetting, mixing and centrifuging, using stocks with different viscosities and densities. Also collecting pellets and separating layers of liquids.
June 17 th
- Stage 1 of DNA purification Link
Comments
The skills include grinding up leaf tissue and extracting the carbohydrate and protein, then concentrating the nucleic acid by precipitation.
June 18 th
- Stage 2 of DNA purification Link
Comments
The skills include selective degradation of RNA and protein, extracting away the residue and collecting the DNA by precipitation.
June 22 nd
- Quality Control of the DNA - Spectrophtometry and Gels Link
- Quality Control of the DNA - PCR Link
Comments
The skills include spectrophotometry and gel electrophoresis of the DNA, and PCR amplification of a chloroplast segment.
June 23 rd
- Restriction Enzyme Digestion and adaptor ligation to create sequencing fragments
- Ampure XP size selection to select fragments of the correct length
Comments
The skills include using resetriction enzymes to fragment the DNA leaving defined ends, adding known short DNA segments to those ends with DNA ligase, and using size selection to acquire fragments you can sequence.
June 24 th
- Ligation of Sequencing primers
- Ampure XP bead selection to remove unused primers
Comments
The skills include the final step of sequencing library production using bar-coded partially matching PCR primers and removal of unused primers with Ampure bead selection.
June 26 th
Field trip to Dr. Weller's lab to use the Illumina MiSeq instrument
- Run samples on the Agilent Bioanalyzer to quantify libraries
- Use the Qubit fluorometer to quantify libraries
- Dilute libraries, pool, add control sequences and load onto a flow cell.
Comments
Prep Sheets - these show what type of preparation we do prior to each class, perhaps useful.
- Week 1 (June 12) Link
- Week 2 (June 15-19) Link
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- Week 3 (June 22-26) Link
