Dr. Jennifer Weller @ UNC Charlotte

Olympic B3 Science Summer Camp 2015 Biotechnology, Biodiversity and Bioinformatics June 12^th - June 26^th
Schedule: (Subject to change)
Date - June	9am-10am	10am-11am	11am-11:30pm	11:30pm-12:00pm	12:00pm-2pm
12^th	Putnam and Smith Getting Acquainted Activity Intro to lab: (1)Establish rules, and set expectations. (2) Lab teams meet at assigned station. (3)Lab equipment described	Smith Pre-Assessment activities Guest: The American Chestnut Foundation (TACF) Treasurer Steve Barilovits: Hybrid trees, Learning Box of Chestnut Materials	Guest: Ranger Kelly Cook Natural History of Crowder's Mountain - Biogeography	Lunch Break	Guest: Ranger Kelly Cook The Chestnut Blight Ms. Smith and Dr. Weller Preparation notes for field trip - collecting/recording team duties
June 15^th - Crowder's Mt State Park Collecting Trip Please arrive at the parking lot by 8:15am Wear proper clothing and bring lots of water, sun screen, and your lunch! If you are traveling separately, please be sure to arrive at the Linwood parking lot by 9:15am. Directions: Take I 85 South (through Gastonia) to Edgewood Road (Exit 13).Take a left (south) and cross over Highway 74. Continue straight.This will be Archie Whitesides Road. Continue until it dead ends into Linwood Rd.Turn left. The Linwood Access and parking area will be on the right - just a short distance down Linwood. Map of Crowders MT SP
16^th	Weller - (1) Overview of lab activities (2)Making Lab solutions - how to read and carry out the instructions	Weller, Smith, Putnam Lab Skills : accuracy and precision when pipetting	Weller, Smith, Putnam Lab Skills: mixing and centrifuging solutions	Lunch Break	Putnam Keeping a laboratory notebook, using the microscope and activities available while waiting for long steps in experiments.
17^th	Weller (1)First steps of the DNA purification method explained and demonstrated	Weller, Smith, Putnam Tissue grinding and incubation Putnam (1) Plant Breeding: Meiosis and Variation	Weller, Smith, Putnam DNA extraction and centrifugation Putnam (2) Computational Activity: Punnett Squares	Lunch Break	Weller, Smith, Putnam Separate phases, precipitate DNA Weller Biotech Careers; Ethics example of the day
18^th	Weller Continuation of DNA purification explained and demonstrated	Weller, Smith, Putnam Pellet DNA, resuspend, remove RNA	Smith Spectrophotometry and dilutions	Lunch Break	Weller, Smith, Putnam Practice dilutions, spectrophotometry, Final DNA precipitation Weller Biotech Careers; Ethics example of the day
June 19^th - Field Trip to Pryor Farm, at 72 Will Hill Road, Hendersonville, NC. Route via US 74 West, I-26 West, and US 64 East. Please arrive at the parking lot by 7:30am at the latest Wear proper clothing and bring lots of water, sun screen, and your lunch! If you are traveling separately, please be sure to check your map and have the cell phone of one of the teachers. Directions from Olympic High School. Head North on Sandy Porter Rd and take the first right onto Arrowood Rd, in 0.4mi turn Left onto 485 Inner North (to Gastonia). Take exit 10 for I-85 toward Gastonia. After 20 miles take exit 10B to get on I74W towards Kings MT. After 56miles merge onto I-26W. After 16 miles take exit 49A to merge onto US-64E, toward the Bat Cave. Turn Right onto Gilliam MT Road, then Left onto Sugarloaf MT Road, first Left onto Ledbetter Rd, which dead ends at Bald Rock Rd, where you take a slight Left. Bald Rock more or less dead-ends - take a Right onto Blythe Stepp Rd, the second left onto Timber Trail and continue onto Will Hill Rd. The destination will be on the right. A Hand-drawn map of the last bit is linked here. PSisco Directions and from Google Maps GoogleMaps Directions
22^nd	Smith How to make sure you have clean DNA: spectrophotometry, gel electrophoresis, PCR	Smith, Putnam Pellet and resuspend DNA, use spectrophotometer Weller PCR overview	Smith, Putnam Lab skills- pour and load agarose gels, set up PCR	Lunch Break	Smith, Putnam Stain agarose gels and make a digital image
23^rd	Weller Restriction Endonuclease digestion and DNA ligase action	Weller, Smith, Putnam Set up RE reactions, Pour and load agarose gels with cp PCR products	Weller, Smith, Putnam Carry out Ampure XP bead size selection	Lunch Break	Weller, Smith, Putnam Stain agarose gels, make a digital image Ligate adaptor sequences (overnight) Weller Biotech Careers; Ethics example of the day
24^th	Weller Making overlapping PCR amplicons for seuqencing	Weller, Smith, Putnam Second PCR with Illumina primers	Weller, Smith, Putnam Ampure bead cleanup, pour agarose gels	Lunch Break	Weller, Smith, Putnam Nanodrop spectrophotometry of sequencing library, confirmatory gels Putnam Plan posters and team demonstrations for Parents Day Weller Biotech Careers; Ethics example of the day.
25^th Student Demonstration Day - 6:30-8:30pm - Please come see our results!!!	Smith Prepare posters of results for parents	Putnam Prepare stations for demos for parents	Erichsen Working in Biotech in North Carolina	Lunch Break	With Guests and Judges: 6:30-8:30pm at Steele Creek Community Room, 13640 Steelecroft Cr. Charlotte, NC 28278 Show your family and friends what you have learned, Celebrate and see certificates awarded Directions
June 26^th Sequencing Lab Field Trip Field trip to the Bioinformatics Building on the UNC Charlotte campus Hosts: Dr. Jennifer Weller and Dr. Cathy Moore Please arrive at the parking lot by 8:15am Wear proper clothing for a research lab, lunch will be provided. If you are traveling separately, please be sure to check your map and have the cell phone of one of the teachers. Directions from Olympic HS to Bioinformatics UNC Charlotte
Presentations		Pictures and Blog		Extra Reading
Web Links		Discussion topics		Lab Protocols

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